Guillaume Gaullier

@GetzlerChem@mstdn.science As I was writing my first answer, I remembered this paper: https://doi.org/10.1038/s41467-023-41444-x Controlled pH change in vitreous ice, with transient melting and rapid re-vitrification to allow a pH-dependent protein conformational change to occur. The very fact that this could work indicates that the initial vitrification did not alter the initial pH too much, at least with this buffer system.

@GetzlerChem@mstdn.science The things being vitrified are macromolecules (proteins, DNA, RNA, by themselves or in complexes) from "small" (~30 kDa, big for a chemist I know) to very big (like whole virus particles). And they are always in an aqueous solution with at least a pH buffer system, a bit of salt, and whatever else is necessary to keep the particles "happy" (membrane proteins need detergents or lipid nanodiscs, etc.). These macromolecules have many solvent-exposed groups that can exchange protons. And in general, they denature at extreme pH values. Denaturation isn't a big problem because if proteins fully unfold, we see it during initial imaging and repeat the prep with new conditions until we see them in their native state. I am thinking more about pH-sensitive regulations: conformational changes caused by changes in pH, protonation state of small-molecule ligands causing them to bind differently, etc. Good point about fluorophores being sensitive to temperature. I know some of them work at cryogenic temps because this is how cryoCLEM works (cryo correlative light and electron microscopy: you localize targets by fluorescence microscopy first, then image at higher magnification in an electron microscope), but it could be that only a few work and the pH probes won't. I was hoping to find some literature about this.

@annakcroft @adredish I think crediting reviewers is good! It's making it one more quantitative metric that is bad.

After I set up human.json on my website yesterday, I figured I also don't post generative AI content on mastodon, and don't use it to code either. So I listed my mastodon and codeberg profiles in my human.json, because why not? It works fine and I like seeing this green dot of the browser extension more often (helps getting used to it!).

@leouieda@bertha.social @gedankenstuecke@scholar.social yes this is very fun! It puts people back in the web, since this green dot means something like "someone you know read this and thinks it meets some standards". Much nicer feeling than wondering why something is algorithmically shown to me.

@albertcardona@mathstodon.xyz @steveroyle@biologists.social Thanks! I added both your sites to my list. Albert, your file is missing commas to separate the items in the "vouches" array, so the browser extension doesn't parse your file (in fact it doesn't even show there is one on your site, I browsed to it directly and Firefox told me about the missing commas).

@gedankenstuecke@scholar.social @leouieda@bertha.social I just listed both of your sites!

@boehmax@mastodon.green oof this seems brutal… what happened to the previous 12 versions? I hope not all desk-rejected without review… Good luck with this one! Hopefully the last. 🤞

@aeryn_thrace@mastodon.social The fact that papers are used to measure researchers against each other for career advancement seems like a big obstacle to the necessary changes you describe, unfortunately. I agree with you that an actionable and collective "knowledge system" is better than a pile of PDFs, but a bigger reform of how academia functions might be required first.

Looks like human.json isn't mentioned yet on the Indieweb wiki. Would anyone with an account there add a page for it? https://indieweb.org

@gabe_sky@infosec.exchange This is super useful, thank you! 🙏 One important improvement would be for you to add a license, so we know what kind of re-use is permitted. For example, can one host a copy on their own website? This is the kind of small but useful tool that would fit well in the online services offered by non-profits like @Framasoft@framapiaf.org and small, local hosting service providers like the ones in the @ChatonsOrg@framapiaf.org collective.

@alberto_cottica@mastodon.green I can see the quoted post's preview. It might depend on how fast difference instances synchronize their posts?

@albertcardona@mathstodon.xyz I hope it will take off! But that will only solve half of the problem. AI crawlers hammering servers is also a big threat...

@miko@piaille.fr J'ai du mal à croire que le cochon ne s'est pas présenté à cette élection. Même quand le loup est suffisamment haut dans les sondages pour nécessiter une alliance de toute la prairie pour le contrer, le cochon est quand même tenté d'ignorer la primaire...